Electrical cell-based biosensors that use microelectrodes as singal transducers have been extensively used in various bio-related studies. A number of methods are available for increasing the biocompatibility of transducers to promote the biological conditions for mammalian cell adhesion and proliferation. Mimicking the extracellular matrix microenvironment using collagen or fibronectin-coated electrodes is one of the most favorable methods; however, high cost and unwanted signal have been identified as common disadvantages of these materials. Here, we introduce graphene oxide as an alternative and effective material for electrode modification. Graphene oxide nanosheets provide oxygen-containing groups and a rough surface, allowing spontaneous adsorption of proteins via a combination of interactions, intensifying cell binding and cell growth. The cellular impedance signal is accordingly more sensitive …​